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KMID : 0380020110260030206
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 3 p.206 ~ p.210
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Effect of Alginate on the Growth of Nannochloropsis oculata NIES-2145
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Park Hyun-Jin
Kim Young-Hwa
Lee Jae-Hwa
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Abstract
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The growth effect of Nannochloropsis oculata (N.oculata), unicellular microalgae, by alginate was investigated. Alginate was depolymerized with sulfuric acid (H2SO4) and heat (121oC), simultaneously. Addition of 0.75% alginate oligomer depolymerized with 0.2 N H2SO4 showed the maximum yield and the growth rate of N. oculata. Chlorophyll content and reducing sugar was increased by alginate oligomer in a dose-dependent manner. Alginate oligomer promoted the growth of N. oculata, whereas the original alginate polysaccharides had no significant effect. Laminaria japonica (L. japonica) extract containing high level of alginate was also increased growth rate and chlorophyll content. CO2 supply addition to L. japonica extract showed no change the growth rate, although addition to alginate oligomer showed prominently increased. N. oculata could use more saccharides in presence of CO2 according to reducing sugar determination. From these results, it is useful to establish optimal condition for high cell density cultivation of N. oculata.
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KEYWORD
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Alginate, Nannochloropsis oculata, Laminaria japonica, CO2
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